Insertion junction: LMJ.RY0402.160315_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.160315
Chromosome:chromosome 4
Location:1250834
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g215150 SSS1B,SSS1,SSIb (1 of 1) IPR023159 - SO1590-like domain; Soluble starch synthase IB intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGGGTTGGATGATGGGAAGTTAGGGCAG
Internal bar code:TGAGCATCGCGCATTGGGCTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:176
LEAP-Seq percent confirming:99.0991
LEAP-Seq n confirming:220
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ATGCAGTGGTCAGGTTAGGG
Suggested primer 2:CACCTTCCCACCTCTTACCA