| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.160353 |
| Chromosome: | chromosome 15 |
| Location: | 447381 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g636450 | SMM58 | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 1) K15190 - 7SK snRNA methylphosphate capping enzyme [EC:2.1.1.-] (MEPCE, BCDIN3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCTCCCTTCCCACCTGCACGCCTTCTT |
| Internal bar code: | CTCGGAGTGAGGACTACCGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 612 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 442 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGTTTGGGAGTGAATGTG |
| Suggested primer 2: | TTCACATAGGACCGCAACAA |