| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.160360 |
| Chromosome: | chromosome 9 |
| Location: | 6134177 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g405300 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCCTCGATGTACACACCTCCTGCTACTC |
| Internal bar code: | AATTTTGTTCCGAAAGGGTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 730 |
| LEAP-Seq percent confirming: | 89.9557 |
| LEAP-Seq n confirming: | 1827 |
| LEAP-Seq n nonconfirming: | 204 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAACTTGGGTTGCCTTCA |
| Suggested primer 2: | GAGGAAGAGGAGTTTGTGCG |