| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.160438 |
| Chromosome: | chromosome_9 |
| Location: | 1259437 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g399400 | FAP199,TGL15 | Triacylglycerol lipase | sense | 3'UTR|3'UTR_intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCTTCTGTAGTCCTACTTGAGTGTATTCAC |
| Internal bar code: | GCTCCGGTCGCCTCGGGAATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1364 |
| LEAP-Seq percent confirming: | 98.4615 |
| LEAP-Seq n confirming: | 192 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGGAAGTGAGTAGGCCG |
| Suggested primer 2: | CTAATGAGGGCCACACCACT |