Insertion junction: LMJ.RY0402.160447_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.160447
Chromosome:chromosome 3
Location:2479617
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g159550 (1 of 7) IPR011989//IPR016024 - Armadillo-like helical // Armadillo-type fold 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCTTTCCTTGGCAACATTTCACGCGTAG
Internal bar code:CCGTAGGGCAGCAGACGGTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:469
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:137
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCTGTAAAAGCGTCTGGC
Suggested primer 2:AGCTCGTCCAGGTTAGTGGA