Insertion junction: LMJ.RY0402.160455_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.160455
Chromosome:chromosome 2
Location:1924675
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g087850 PTK19 Protein tyrosine kinase; (1 of 2) 2.7.10.2//2.7.11.25 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase // Mitogen-activated protein kinase kinase kinase / MLTK 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTTTTGAAAGACTCTTCATGCAAGCGGC
Internal bar code:TCGCGGGTCCGAACGGTCGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:925
LEAP-Seq percent confirming:99.9177
LEAP-Seq n confirming:1214
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTAAATGGAATGGGAATG
Suggested primer 2:CAAGCACAACACCCATTCAC