| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.160456 |
| Chromosome: | chromosome 17 |
| Location: | 3962949 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728700 | (1 of 1) K11228 - mitogen-activated protein kinase kinase kinase (STE11) | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGTCGGAAGAGAGCGGGATGCGAGGGA |
| Internal bar code: | ACCGGGCCACTCGGACTCCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 382 |
| LEAP-Seq percent confirming: | 89.8734 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTTGCGACTTCCTTGTCA |
| Suggested primer 2: | AGCTGGGCCTGCGTATAGTA |