| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.160464 |
| Chromosome: | chromosome_4 |
| Location: | 1608435 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g213251 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTGTTTGAAAGGTGGGTTTGGGGGTGTTG |
| Internal bar code: | GGACACACGCGGGCTAACCCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 963 |
| LEAP-Seq percent confirming: | 99.6816 |
| LEAP-Seq n confirming: | 2818 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTACTGAGACGGAGGAAC |
| Suggested primer 2: | CAACGAGGTTGGAAATGGTT |