| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.160494 |
| Chromosome: | chromosome 6 |
| Location: | 8376348 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g306750 | FAP290 | Flagellar Associated Protein 290; (1 of 1) K13448 - calcium-binding protein CML (CML) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAAGGGTTAAGGAACCCTACCCCAACCC |
| Internal bar code: | ATCTGGTACCGTCGCTCCCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 432 |
| LEAP-Seq percent confirming: | 99.9061 |
| LEAP-Seq n confirming: | 1064 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGCTGTCGAAGAACATGA |
| Suggested primer 2: | AAAAGGAAACCCCTGAGGAA |