| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.160510 |
| Chromosome: | chromosome 3 |
| Location: | 5373763 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g184500 | (1 of 2) PTHR33925:SF3 - PROTEIN ACCUMULATION AND REPLICATION OF CHLOROPLASTS 6, CHLOROPLASTIC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGATGCCCCAATGATACGGTATGCGATA |
| Internal bar code: | AGCGGGTAGTGTCAGGAGCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 357 |
| LEAP-Seq percent confirming: | 98.9817 |
| LEAP-Seq n confirming: | 8554 |
| LEAP-Seq n nonconfirming: | 88 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTTTTCCTGCCGCCTATT |
| Suggested primer 2: | GGACCAGAGGTTATGACGGA |