Insertion junction: LMJ.RY0402.160530_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.160530
Chromosome:chromosome 16
Location:5966716
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g676800 (1 of 1) PTHR11017//PTHR11017:SF145//PTHR11017:SF158//PTHR12203//PTHR12203:SF13 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED // SUBFAMILY NOT NAMED // KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED // F10K1.7 PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCCCTACCCAGCACCCACCGCTTCCTCGC
Internal bar code:CTGATGGTTGTCGTCACACACG

Confirmation - LEAP-Seq

LEAP-Seq distance:660
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTAGAGGTTCAGGTTGGG
Suggested primer 2:GCCTGAACCCTCTCTCTCCT