| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.160545 |
| Chromosome: | chromosome 6 |
| Location: | 322847 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g251300 | SMP6B | U6 snRNA-associated Sm-like small nuclear riboprotein LSm4 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACGTCCAGGCTCCTGGTAGCTTGTAAGG |
| Internal bar code: | CGACTTGAGCATTTAGCCTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 247 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 113 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGCTTGTGGTACCTGAC |
| Suggested primer 2: | GCATCTTAAAGAACCGCTGC |