Insertion junction: LMJ.RY0402.160634_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.160634
Chromosome:chromosome 9
Location:1112611
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g400312 (1 of 5) IPR001680//IPR011047//IPR017986 - WD40 repeat // Quinonprotein alcohol dehydrogenase-like superfamily // WD40-repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGTCTATCAAAGCCCCCACCGACACGGCC
Internal bar code:TGTACCGGGTTACATGAGTCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:217
LEAP-Seq percent confirming:85.4749
LEAP-Seq n confirming:153
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTTCTCCTGACACACAGG
Suggested primer 2:CGCCAGAAGTAGAGGTCGAG