Insertion junction: LMJ.RY0402.160643_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.160643
Chromosome:chromosome 1
Location:5729412
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g040850 (1 of 1) IPR002048//IPR003368//IPR006626//IPR010308//IPR011050 - EF-hand domain // Polymorphic outer membrane protein repeat // Parallel beta-helix repeat // TRP-like family // Pectin lyase fold/virulence factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGGTGGCGCTGCTGGGCAGACCCGTGGC
Internal bar code:CCTTTTTGAACGATTTGCCCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:549
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCTATGCCGTTGACATTG
Suggested primer 2:TTGCTGCTGCTACTGCTGTT