| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.160660 |
| Chromosome: | chromosome 7 |
| Location: | 1474602 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g323600 | (1 of 1) PF11317 - Protein of unknown function (DUF3119) (DUF3119) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGCAGGCCTAGCTGCAGGTTGAACGAGG |
| Internal bar code: | GGAGATCGTCCCGCGGAGTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 747 |
| LEAP-Seq percent confirming: | 99.9114 |
| LEAP-Seq n confirming: | 1128 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGTATTCCAATCCCAAGG |
| Suggested primer 2: | TAACATTTCGGAGACTGCCC |