| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.160680 |
| Chromosome: | chromosome 3 |
| Location: | 5752450 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g188350 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAGGTGGGCCGGGCGGTGTTTATGTGCT |
| Internal bar code: | GACACGATGCGGTGCTCCGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 618 |
| LEAP-Seq percent confirming: | 95.5959 |
| LEAP-Seq n confirming: | 5318 |
| LEAP-Seq n nonconfirming: | 245 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACCGACAAGATAGGTGGT |
| Suggested primer 2: | CCCAAACCTATCTGCTCCAA |