| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.160681 |
| Chromosome: | chromosome 3 |
| Location: | 1838375 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g154600 | (1 of 2) PTHR35514:SF1 - THYLAKOID LUMENAL 15.0 KDA PROTEIN 2, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCCGCCCGTTGGTGCACGTACGACACGA |
| Internal bar code: | ACTGCGAATTACCATCCTGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 463 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 501 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGCATCCACGACTAAAG |
| Suggested primer 2: | GTTTGTTTGGGAGGAGGACA |