Insertion junction: LMJ.RY0402.160707_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.160707
Chromosome:chromosome 17
Location:6107107
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g741400 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCTGCTCGTGGGTATGTGGGTGTGTGGT
Internal bar code:CGGGCCCATCTCAACCCTTCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:739
LEAP-Seq percent confirming:85.598
LEAP-Seq n confirming:3156
LEAP-Seq n nonconfirming:531
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:ATGAACTGGTTACCCACCCA
Suggested primer 2:AACAGTGGCATCAGCAACAC