| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.160723 |
| Chromosome: | chromosome 1 |
| Location: | 1015778 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g005813 | POB31 | Proteome of basal body 31 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAAGCGAACCGGGGGAGGGGGCGCCGTA |
| Internal bar code: | GTCACGTCCTACAAAAAGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 799 |
| LEAP-Seq percent confirming: | 99.9313 |
| LEAP-Seq n confirming: | 1454 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCGGAGCCCATGTAACAC |
| Suggested primer 2: | TAGCTTGGCTGTCGCCTTAT |