| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.160725 |
| Chromosome: | chromosome_14 |
| Location: | 2308160 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g623439 | antisense | 3'UTR|intron|outside_mRNA |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AATATGTAGGGGGGCAGCATGCAGCAGGCG |
| Internal bar code: | TTCTTCGACAGAAGTGTTTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 369 |
| LEAP-Seq percent confirming: | 99.7434 |
| LEAP-Seq n confirming: | 3498 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGACTACTACCGCGTGTGC |
| Suggested primer 2: | CACCACCTACCGTCTCGAAT |