| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.160729 |
| Chromosome: | chromosome 2 |
| Location: | 6719076 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g118100 | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCTAACACCCGACAGAGCAGTCACAGCC |
| Internal bar code: | CTAACGGGTCTGCGTGATCTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 781 |
| LEAP-Seq percent confirming: | 99.4169 |
| LEAP-Seq n confirming: | 682 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTGTGGTGTAGGCGTTT |
| Suggested primer 2: | GTTGGATCGGGCACTGTACT |