| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.160741 |
| Chromosome: | chromosome 5 |
| Location: | 1656138 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g243050 | TRX3,TRXf2,TRXF2 | (1 of 1) PTHR10438:SF258 - THIOREDOXIN F1, CHLOROPLASTIC-RELATED; Thioredoxin f2, chloroplastic | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGTGGTGGACTTCTACACCGTGAGTGAC |
| Internal bar code: | GGACAGGTCCGAGGACGCGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 29.1667 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 119 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCCGTCTCTCCGTCTTGT |
| Suggested primer 2: | TGGTACGTCTCCAAATGGGT |