| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.160749 |
| Chromosome: | chromosome 16 |
| Location: | 4756381 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686650 | (1 of 1) PTHR31057//PTHR31057:SF0 - FAMILY NOT NAMED // E3 UFM1-PROTEIN LIGASE 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGTGCGCGGGAGACTATCGCCAACACA |
| Internal bar code: | TTGTGTCTCGGAGCGCGGCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 179 |
| LEAP-Seq percent confirming: | 99.3561 |
| LEAP-Seq n confirming: | 5864 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGAAGGGTGAGGAGGAGC |
| Suggested primer 2: | CGAACCCTCTCCACTCTCAC |