Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.160755 |
Chromosome: | chromosome_6 |
Location: | 2219025 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g266250 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GAGCTGCACAGGGCCATATGCAAAAGCCCG |
Internal bar code: | CATGGGTTTGTCCTACGCGCAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 544 |
LEAP-Seq percent confirming: | 99.7085 |
LEAP-Seq n confirming: | 342 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TACGGGGGATCAGAGTCTTG |
Suggested primer 2: | CTTTTGGGCTTTCAGCTTTG |