| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.160755 |
| Chromosome: | chromosome 6 |
| Location: | 2219025 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g266250 | (1 of 15) PTHR12291:SF23 - PROTEIN MEC-15 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCTGCACAGGGCCATATGCAAAAGCCCG |
| Internal bar code: | CATGGGTTTGTCCTACGCGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 544 |
| LEAP-Seq percent confirming: | 99.7085 |
| LEAP-Seq n confirming: | 342 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGGGGGATCAGAGTCTTG |
| Suggested primer 2: | CTTTTGGGCTTTCAGCTTTG |