Insertion junction: LMJ.RY0402.160755_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.160755
Chromosome:chromosome 8
Location:3806884
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g377350 ADK5,FAP180,FAP121,FAP18 (1 of 1) 2.7.4.3//2.7.4.8 - Adenylate kinase / Myokinase // Guanylate kinase / Guanosine monophosphate kinase; Flagellar Associated Protein 180 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACGTCACGTCCGCGTTACTAGATGCTGTC
Internal bar code:TACGGTGGAGGTGTTCGAACCT

Confirmation - LEAP-Seq

LEAP-Seq distance:425
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:52
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TGAGTGCATCTGTGCTTTCC
Suggested primer 2:GGTGCTGAAAAGCAAGGAAG