| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.160766 |
| Chromosome: | chromosome 6 |
| Location: | 8425410 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g307150 | AMB1,AMYB1 | Beta-amylase 1; (1 of 3) 3.2.1.2 - Beta-amylase / Saccharogen amylase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATGGCAAAGCCCGCCGTCGCGCAGCCAA |
| Internal bar code: | GTTTGCTCGGGCAGAAGCAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCCTAACAGCAATGAGGG |
| Suggested primer 2: | ATTTGCAAGTCGTCCTCCAC |