| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.160828 |
| Chromosome: | chromosome 3 |
| Location: | 4793132 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g178550 | (1 of 1) K10260 - F-box and WD-40 domain protein 7 (FBXW7, SEL10) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACAGCCCCGAGCCCCGAGGTCGTATCAT |
| Internal bar code: | GGTTTGCTGGGAACGGGAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 777 |
| LEAP-Seq percent confirming: | 99.7729 |
| LEAP-Seq n confirming: | 1757 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCAACTTAGGGATGGGA |
| Suggested primer 2: | CTTTCACGTAAACCCGCAAT |