Insertion junction: LMJ.RY0402.160828_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.160828
Chromosome:chromosome 8
Location:855759
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre08.g361150 GT90F11,GT90-11 GT90 family protein 11; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACACACACATACATACACACACAGGA
Internal bar code:CTCAACCGGCCCACTCGGAGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:652
LEAP-Seq percent confirming:42.3287
LEAP-Seq n confirming:1705
LEAP-Seq n nonconfirming:2323
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTCCACTTCAACCTCTTC
Suggested primer 2:TAAACTGTGACCCAGGGAGG