| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.160852 |
| Chromosome: | chromosome 6 |
| Location: | 3472319 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278097 | RAE1 | mRNA export protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCAAAGCTGGCACAGGGTCCACGGGACT |
| Internal bar code: | TAGCCACAGCGGCTACGAGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1058 |
| LEAP-Seq percent confirming: | 99.7957 |
| LEAP-Seq n confirming: | 5861 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGTGTAAGGAGGACGAGG |
| Suggested primer 2: | GCTTTGTGGAGCAAGCCTAC |