| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.160853 |
| Chromosome: | chromosome 11 |
| Location: | 2847016 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g477625 | CHLH2,MCH2 | Magnesium chelatase subunit H; (1 of 1) PTHR23304//PTHR23304:SF109 - SPOT2-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGGTTAGTGGGGGAGGGACAGGCGACTT |
| Internal bar code: | GTGTGCTTGAGCACACTTCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 168 |
| LEAP-Seq percent confirming: | 99.7113 |
| LEAP-Seq n confirming: | 2418 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAACAAACACCAATGCAC |
| Suggested primer 2: | GAAAGACTCGCCTCCTGTTG |