Insertion junction: LMJ.RY0402.160855_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.160855
Chromosome:chromosome 2
Location:5768095
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g110650 (1 of 83) IPR029044 - Nucleotide-diphospho-sugar transferases intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGACCGAGTCCAGCTTCCCAAATCGGCAA
Internal bar code:TTCGCAAGTTCTGACTGCGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:70
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTTACCCTCTGTAGCTGG
Suggested primer 2:CGGTAAAATGGTGGGTATCG