| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.160858 |
| Chromosome: | chromosome 12 |
| Location: | 2998356 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g501050 | ALDH25,ALD6 | Aldehyde/histidinol dehydrogenase; (1 of 1) 1.1.1.1//1.2.1.10 - Alcohol dehydrogenase / Aldehyde reductase // Acetaldehyde dehydrogenase (acetylating) / Aldehyde dehydrogenase (acylating) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCATCAAAGTTCGGGCACGCAAAAGTGTG |
| Internal bar code: | GTACATACGTAATTGCATCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 828 |
| LEAP-Seq percent confirming: | 99.259 |
| LEAP-Seq n confirming: | 2411 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCTAAGCCCTTGTTCACG |
| Suggested primer 2: | GCAAATAGAGGAGCGAGTGG |