| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.160863 |
| Chromosome: | chromosome_13 |
| Location: | 232684 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g563700 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGTGTGTGTGTGTGGGGGGGGATAATTGCC |
| Internal bar code: | GACTAGTCCGGGCGTTGGCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 256 |
| LEAP-Seq percent confirming: | 86.9043 |
| LEAP-Seq n confirming: | 2462 |
| LEAP-Seq n nonconfirming: | 371 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGGAACATGGACGACACA |
| Suggested primer 2: | ACATACTGAGAACCCGGACG |