| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.160866 |
| Chromosome: | chromosome 2 |
| Location: | 8560504 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g141946 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTGGCAGCGGGCACACCCACAAAAGAT |
| Internal bar code: | GTTACGGCGCGTTTCAGGCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 82 |
| LEAP-Seq percent confirming: | 70.3863 |
| LEAP-Seq n confirming: | 164 |
| LEAP-Seq n nonconfirming: | 69 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAATGTCGGACTACGGAT |
| Suggested primer 2: | AGTGGAGTAGGCCAGCTGAA |