Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.160870 |
Chromosome: | chromosome 11 |
Location: | 1539218 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g467752 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCCGAGAGCCCCAACCCAGAGCCCTGCC |
Internal bar code: | CCAGCGATGCTGGATAATAACG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 131 |
LEAP-Seq percent confirming: | 99.7908 |
LEAP-Seq n confirming: | 477 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTCTAGCGTCACACGACACC |
Suggested primer 2: | TATGCCGCACACACATTTTT |