| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.160877 |
| Chromosome: | chromosome 2 |
| Location: | 5645633 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g109550 | HSD1,AHD3 | Homoserine dehydrogenase; (1 of 2) K12524 - bifunctional aspartokinase / homoserine dehydrogenase 1 (thrA) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAATTTTACACCCGTTGATTGCGGAATA |
| Internal bar code: | TTAAGCCCTCGGTAGTTTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 520 |
| LEAP-Seq percent confirming: | 99.1532 |
| LEAP-Seq n confirming: | 2693 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATGTTTGGGCGATGAGGT |
| Suggested primer 2: | TAAAGCAGCCGCGATTTAGT |