| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.160883 |
| Chromosome: | chromosome 13 |
| Location: | 1375102 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g571800 | (1 of 4) PF07059 - Protein of unknown function (DUF1336) (DUF1336) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCGAGTCGTGCCGTGCCGTGCCTTGCCA |
| Internal bar code: | GGTTTTTACCTGGAGCTTGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 626 |
| LEAP-Seq percent confirming: | 88.3238 |
| LEAP-Seq n confirming: | 33155 |
| LEAP-Seq n nonconfirming: | 4383 |
| LEAP-Seq n unique pos: | 78 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGACCTTTGAAAACCAGAC |
| Suggested primer 2: | AGGAAGGTTCTGGTGTGGTG |