| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.160889 |
| Chromosome: | chromosome 3 |
| Location: | 3083944 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g164600 | PMA3,PMH1,ACA3 | (1 of 3) K01535 - H+-transporting ATPase (E3.6.3.6); P-type ATPase/cation transporter, plasma membrane | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCACGAGGAGAAGCGTGATTTCGTTATC |
| Internal bar code: | GAGCGAGGCGAGGGAAGGCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 879 |
| LEAP-Seq percent confirming: | 99.7863 |
| LEAP-Seq n confirming: | 934 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGACCCACACTTTCACACA |
| Suggested primer 2: | ACTTGCTTGCAGCAGGTTTT |