Insertion junction: LMJ.RY0402.160894_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.160894
Chromosome:chromosome 9
Location:5129357
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g398845 (1 of 1) PF08507 - COPI associated protein (COPI_assoc) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTAGGATGCATGTGAGCCAGGGTTTGCTG
Internal bar code:GCGGCGAAGCCTCCCGAAGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:947
LEAP-Seq percent confirming:99.8861
LEAP-Seq n confirming:877
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCTTTTACGCCTCGTGTC
Suggested primer 2:AGCGTCAGAGCAAACTGGAT