| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.160899 |
| Chromosome: | chromosome 8 |
| Location: | 3382562 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g374300 | CGL115 | (1 of 1) K19323 - ataxin-10 (ATXN10); Conserved in the Green Lineage | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAAGCCTGGGGGCGTGCGGCAGCGGAGAA |
| Internal bar code: | GTGCGCACGGTCGCAAGCGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1027 |
| LEAP-Seq percent confirming: | 99.8277 |
| LEAP-Seq n confirming: | 2317 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCATGAAAAGAGGCGTGT |
| Suggested primer 2: | AAGTCTGCAACACCCAAACC |