| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.160914 |
| Chromosome: | chromosome_2 |
| Location: | 84654 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g073750 | KIN9-2,KLP1 | Kinesin motor protein, kinesin-9 (Kif9) family | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTTTGACAGTAGGAAGCTCGTGAGCGTGT |
| Internal bar code: | GCGGTGGCATGGCGGTGTGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1053 |
| LEAP-Seq percent confirming: | 99.4921 |
| LEAP-Seq n confirming: | 1763 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAATGATTGAAACAGCCCG |
| Suggested primer 2: | CCTCACCCTAGCTTGTCAGC |