| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.160930 |
| Chromosome: | chromosome 5 |
| Location: | 2712871 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236802 | (1 of 71) IPR016181 - Acyl-CoA N-acyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACTACGCGGCTGCCGCAGCCTACCAGT |
| Internal bar code: | TATGGGCATGTCACCGCGTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 216 |
| LEAP-Seq percent confirming: | 98.8365 |
| LEAP-Seq n confirming: | 1529 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGGCCTTAGACAGGGCAT |
| Suggested primer 2: | ACTATCCACTGCCACCGAAC |