| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.160958 |
| Chromosome: | chromosome 1 |
| Location: | 4249401 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g028450 | (1 of 1) PTHR30544//PTHR30544:SF2 - 23S RRNA METHYLTRANSFERASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGATGCTGCCTGTTCTTCCAGACCCTTCC |
| Internal bar code: | TGTGTCTCGGTTCGGAGCAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 85 |
| LEAP-Seq percent confirming: | 98.7395 |
| LEAP-Seq n confirming: | 235 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCTCGCATAGAGCTGGTC |
| Suggested primer 2: | AGCTGCCGTAGGAACACACT |