| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.160959 |
| Chromosome: | chromosome 13 |
| Location: | 2582086 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g581050 | GT90F46,GT90-46 | (1 of 11) 2.4.2.26 - Protein xylosyltransferase / Uridine diphosphoxylose-protein xylosyltransferase; GT90 family protein 46 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAACGACCCCACCTCAGGTGTTGCGCCCA |
| Internal bar code: | CCCACAAGATAATCCGTCACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 682 |
| LEAP-Seq percent confirming: | 99.5451 |
| LEAP-Seq n confirming: | 4377 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAAAACGCTGGTCAAAGC |
| Suggested primer 2: | GAGCAAGGGCGAAGTATCTG |