| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.160966 |
| Chromosome: | chromosome 11 |
| Location: | 3272875 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g480000 | (1 of 2) PF02845 - CUE domain (CUE) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCGGTGCCGAGAGCAAATGTCCACTGCA |
| Internal bar code: | ATCCTGGGTGGCGACGTTCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 243 |
| LEAP-Seq percent confirming: | 99.5702 |
| LEAP-Seq n confirming: | 695 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCTCCGATATCGTCAGTA |
| Suggested primer 2: | TCCTACTCCCTGGCAACAAC |