| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.160993 |
| Chromosome: | chromosome 11 |
| Location: | 1299437 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467711 | (1 of 3) PTHR30612 - SECA INNER MEMBRANE COMPONENT OF SEC PROTEIN SECRETION SYSTEM | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTATGACACACACACACACACACACACACA |
| Internal bar code: | GGCGCCAGAAGTGTGCTGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 588 |
| LEAP-Seq percent confirming: | 26.3724 |
| LEAP-Seq n confirming: | 2402 |
| LEAP-Seq n nonconfirming: | 6706 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTTGGTTTGGTTTGGTT |
| Suggested primer 2: | AGTTGGTTGGCGGTTAGTTG |