Insertion junction: LMJ.RY0402.161000_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.161000
Chromosome:chromosome 10
Location:973700
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g424775 CTP2,PCH1,PAA1 Copper transporting ATPase and Cu chaperone for CTP1; (1 of 1) PTHR24093:SF124 - COPPER-TRANSPORTING ATPASE PAA1, CHLOROPLASTIC 3'UTR|outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTATCTGTTCGGTCGCCAGGTGCCTTCCG
Internal bar code:TAAGCTGTGAGTGGTGCGAAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:453
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CTATATCCTCAGGTCGCGGA
Suggested primer 2:GTGTCAGTGCCAGACAATGG