Insertion junction: LMJ.RY0402.161060_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.161060
Chromosome:chromosome 14
Location:2196515
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g622700 UMAMIT (Usually Multiple Acids Move In and Out Transporters) type transporter; (1 of 5) PTHR11132//PTHR11132:SF101 - SOLUTE CARRIER FAMILY 35 // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGCCTAGAATGGTGAAGCGGTGCTCCAT
Internal bar code:TTTTACGTAGCGGGGACGACAC

Confirmation - LEAP-Seq

LEAP-Seq distance:782
LEAP-Seq percent confirming:99.8885
LEAP-Seq n confirming:896
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CGGAGAAAGTGGAAGAGTGC
Suggested primer 2:TTGTCAAGGAACTGTCGCTG