| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.161062 |
| Chromosome: | chromosome 2 |
| Location: | 6020962 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g112400 | CHI1 | (1 of 1) K12310 - Di-N-acetylchitobiase (CTBS); Chitinase-like hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGATTGGGGCAGTGGAGAGCACGTCTTT |
| Internal bar code: | ACCCCAGTGTCGGCACTGGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 989 |
| LEAP-Seq percent confirming: | 94.7761 |
| LEAP-Seq n confirming: | 127 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCCTCCCATAAGAGCAAA |
| Suggested primer 2: | GTGCATGTATTGCAGATGGG |