| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.161115 |
| Chromosome: | chromosome 3 |
| Location: | 2637654 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g160900 | (1 of 1) PF07714//PF12796//PF13857 - Protein tyrosine kinase (Pkinase_Tyr) // Ankyrin repeats (3 copies) (Ank_2) // Ankyrin repeats (many copies) (Ank_5) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTGACGCGCAGTGAATTTGGTTGCCTGC |
| Internal bar code: | ATGTAGGGTTGCTCGTTTTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 353 |
| LEAP-Seq percent confirming: | 99.8909 |
| LEAP-Seq n confirming: | 1832 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGTAGACCTCGGTGTGGT |
| Suggested primer 2: | CACGTGTCCCGATGTGTAAC |